A Quarter Ellipsoidal Reflector Enhancing Fluorescence Collection Efficiency For Multiphoton Microscopes
نویسندگان
چکیده
منابع مشابه
Maximizing fluorescence collection efficiency in multiphoton microscopy
Understanding fluorescence propagation through a multiphoton microscope is of critical importance in designing high performance systems capable of deep tissue imaging. Optical models of a scattering tissue sample and the Olympus 20X 0.95NA microscope objective were used to simulate fluorescence propagation as a function of imaging depth for physiologically relevant scattering parameters. The sp...
متن کاملA laser-induced fluorescence biosensor by using ellipsoidal reflector
A new and compact fluorescence biosensor is constructed. The distinguished feature of this setup is the use of an ellipsoidal reflector as an optical collector of the fluorescence light. A traditional collecting lens with numerical aperture of 0.5 can only collect a few percent of the available signal, while the incorporation of the ellipsoidal reflector enables the apparatus to provide a signa...
متن کاملImaging properties of high aperture multiphoton fluorescence scanning optical microscopes
A theory for multiphoton fluorescence imaging in high aperture scanning optical microscopes employing finite sized detectors is presented. The effect of polarisation of the fluorescent emission on the imaging properties of such microscopes is investigated. The lateral and axial resolutions are calculated for one-, twoand three-photon excitation of p-quaterphenyl for high and low aperture optica...
متن کاملSilica nanoparticles as a tool for fluorescence collection efficiency enhancement
In this work we demonstrate enhancement of the fluorescence collection efficiency for chlorophyll-containing photosynthetic complexes deposited on SiO2 spherical nanoparticles. Microscopic images of fluorescence emission reveal ring-like emission patterns associated with chlorophyll-containing complexes coupled to electromagnetic modes within the silica nanoparticles. The interaction leaves no ...
متن کاملCalibration of Fluorescence Microscopes
We propose a new durable multi-dimensional ruler for the calibration and the alignment of fluorescence microscopes (wide-field, confocal, multiphoton), thanks to non-photo-bleaching fluorescent sub-micrometer patterns embedded in glass in three dimensions. This new and robust tool enables the measurement of stage repositioning, detector’s features, illumination homogeneity, both lateral and axi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: IEEE Photonics Journal
سال: 2016
ISSN: 1943-0655
DOI: 10.1109/jphot.2016.2615284